Main properties of urease partially purified from seeds of Syrian mesquite (Prosopis farcta)
نویسنده
چکیده
Novel sources of urease from leguminosae were selected for activity test, one the them (Prosopis farcta) showed high specific activity (36.52 U mg). The active uerase extract then subjected to series of purification steps involved salting out, DEAE, CM ion exchange and size exclusion chromatography. Specific activity were 52.44, 193.27, 230.55, 433.33 U mg respectively. Main features of urease were detected included estimation of molecular weight (95.500 kDa), optimum pH for activity (8) and stability (7-8), optimum temperature for activity (45C) and stability (25-30 C).
منابع مشابه
اکوفیزیولوژی جوانهزنی بذر علفهرز کهورک (.Prosopis farcta L)
Mesquite is an invasive and problematic weed in warm and dry areas and so its management is very important. In order to study the effective factors on mesquite seed germination, the following studies were conducted to investigate: 1- seed viability; 2- the effect of constant temperatures on seed germination including 5, 10, 15, 20, 25, 30, 35 and 40°C independent dark and independent light ...
متن کاملPhytochemical investigations and antioxidant activity of Prosopis farcta from South Khorasan
Background and objectives: The aim of this study was the phytochemical screening and determining the total antioxidant activity of prosopis. farcta a plant native to South Khorasan. It has been mentioned as a cure for diarrhea, measles, diabetes and some other diseases in home remedies. Neuroprotective and relaxing effects, increasing HDL, decreasing LDL and accelerati...
متن کاملLinkage Analysis in a Heptasaccharide from Prosopis juliflora by Methylation and Periodate Oxidation Methods
Prosopis juliflora (Sw.) DC. commonly known as mesquite, vilayti babul, vilayti kikar belongs to family leguminosae sub family (mimosoidae). It’s a thorny ever-green to semi-evergreen fast growing tree capable of growing in arid and semi-arid regions and attaining heights up to 10m. A polysaccharide was isolated from Prosopis juliflora seed and purified. Purified juliflora polysaccharide has be...
متن کاملDietary and Parasitological Analysis Ofcoprolites Recovered from Mummy 5,ventana Cave, Arizona
Four coprolites were excavated with Burial 5 at Ventana Cave. a partially mummified five-year-old child. Two coprolites were granular and dark in color and two were fibrous and light in color. The coprolites are remains of the child's intestinal contents and were submitted for dietary and parasitological analysis. No parasites were found. The fibrous coprolites proved to be remains of highly ma...
متن کاملPhytophagous insects associated with the reproductive structures of mesquite (Prosopis spp.) in Argentina and their potential as biocontrol agents in South Africa
Mesquites (Prosopis spp.) are thorny leguminous shrubs or trees native to Southwest Asia, Africa, and predominantly North and South America. Introduced as beneficial plants to India, Pakistan, South Africa, Egypt, Kuwait, Australia, U.S.A. (Hawaii), and Brazil, some Prosopis species have become invasive in some of these countries. Mechanical and chemical control methods implemented in the U.S.A...
متن کامل